preparation of modified BG11 medium without any precipitation:
when i prepare the BG11 medium i used to get precipitates and my culture doesn't grow with the medium what i have prepared. now i got the right composition for the medium and the modified one and am posting it here.
when i prepare the BG11 medium i used to get precipitates and my culture doesn't grow with the medium what i have prepared. now i got the right composition for the medium and the modified one and am posting it here.
S.no
|
Chemical Name
|
Quantity (mg/Litre)
|
1
|
Boric
acid
|
2.86
|
2
|
Calcium
chloride Anhydrous
Or
Calcium
Chloride Dihydrate
|
27.18
36
|
3
|
Citric
Acid Anhydrous
or
Citric
Acid.H2o
|
6.00
6.56
|
4
|
Cupric
Sulphate
|
0.079
|
5
|
Ferric
Ammonium Citrate
|
6.00
|
6
|
Magnesium
sulphate.7H20
or
Magnesium
Sulphate
|
75.00
36.658
|
7
|
Manganese
Chloride.4H2o
Or
Manganese
Chloride
|
1.81
1.151
|
8
|
EDTA
|
1.00
|
9
|
Potassium
phosphate dibasic
|
40.00
|
10
|
Sodium
Carbonate Anhydrous
|
20.00
|
11
|
Sodium
Molybdate.2H20
or
Sodium
Molybdate
|
0.39
0.338
|
12
|
Zinc
sulphate.7H20
or
Zinc
Sulphate
|
0.222
0.1247
|
13
|
Cocl2.6H20
|
50
|
Properties
of medium:
Form:
liquid
Appearance:
light yellow to yellow
Solubility:
Miscible with water
Storage:
2-6’C
PH:
8.0 (by adding the NaOH or KOH)
* please avoid autoclaving the medium for long time because it leads to precipitate, pressure of about 15 to 20 pounds for 45 minutes
Congratulations Madhu. Keep up ur hard work.
ReplyDeletethank you very much...
Deletevery nice to hear such recipe for your lab!
Deletegood keep it up!
and we expect more from you!
very nice to hear such a recipe for your lab!
Deletegood keep it up!
and we expect more from you.
Great to see your post and I like the smily photograph exuding satisfaction. This is all about persistence and hard work, keep it up..
ReplyDeletethank u very much mam for affording me the opportunity...
DeleteThank God!! Good attempt madhu!
ReplyDeleteyea... dips.. thank u
DeleteThanks for info on feedstock for astaxanthin production.I have been using a big inland phytoplanktondeposit,more Elementally complete shows in feeding behavior of bugs and fish.
ReplyDelete