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Friday, April 19, 2013

preparation of modified BG 11 medium

preparation of modified BG11 medium without any precipitation:

when i prepare the BG11 medium i used to get precipitates and my culture doesn't grow with the medium what i have prepared. now i got the right composition for the medium and the modified one and am posting it here.


S.no
Chemical Name
Quantity (mg/Litre)
1
Boric acid
2.86
2
Calcium chloride Anhydrous
Or

Calcium Chloride Dihydrate
27.18


36
3
Citric Acid Anhydrous

or
Citric Acid.H2o
6.00


6.56
4
Cupric Sulphate
0.079
5
Ferric Ammonium Citrate
6.00
6
Magnesium sulphate.7H20

or

Magnesium Sulphate
75.00



36.658
7
Manganese Chloride.4H2o

Or

Manganese Chloride
1.81



1.151
8
EDTA
1.00
9
Potassium phosphate dibasic
40.00
10
Sodium Carbonate Anhydrous
20.00
11
Sodium Molybdate.2H20
or
Sodium Molybdate
0.39

0.338
12
Zinc sulphate.7H20
or
Zinc Sulphate
0.222

0.1247

13
Cocl2.6H20
50 

Properties of medium:
Form: liquid
Appearance: light yellow to yellow
Solubility: Miscible with water
Storage: 2-6C
PH: 8.0 (by adding the NaOH or KOH)
* please avoid autoclaving the medium  for long time because it leads to precipitate, pressure of about 15 to 20 pounds for 45 minutes




9 comments:

  1. Congratulations Madhu. Keep up ur hard work.

    ReplyDelete
    Replies
    1. very nice to hear such recipe for your lab!
      good keep it up!
      and we expect more from you!

      Delete
    2. very nice to hear such a recipe for your lab!
      good keep it up!
      and we expect more from you.

      Delete
  2. Great to see your post and I like the smily photograph exuding satisfaction. This is all about persistence and hard work, keep it up..

    ReplyDelete
    Replies
    1. thank u very much mam for affording me the opportunity...

      Delete
  3. Thank God!! Good attempt madhu!

    ReplyDelete
  4. Thanks for info on feedstock for astaxanthin production.I have been using a big inland phytoplanktondeposit,more Elementally complete shows in feeding behavior of bugs and fish.

    ReplyDelete