why sequencing of genome?
each and every gene should be studied and the function of the gene, operons, the base pare composition also should be known for the entire genome or the entire chromosome. the genome sequencing also be useful for personalized medicine also for curing the diseases also by sequencing the genome. the first sequenced whole genome was H. Influenza and the bacteriophage MS2 . early the genome sequencing was done by Maxam gilbert and sangers method, well it is very popular method but its a tedious method,as the time changes the science also improves and grows fast and so many sequencing technologies where growing. the recent one is the next generation sequencing method.
The one method which we are interested is SOLID lets see about the method and how its done ,
Its introduced by the Life technologies it is one of the method and here, the sequencing is done by the DNA ligase and it leads to the formation of beads with the adapter each beads contains the same copies of DNA and those base pairs are further used, and the each base are represented in 4 different colors. then will get so much of data then the first step is assembly of data. we first clean or remove the unwanted data(remove vector adaptor) and the sequencing give the so many short fragments of the overlaps called contigs and those contigs may be the size of 4-5kb in. then the scaffolds are formed and we can find the size of the scaffolds by using the BAC insertion size. the assembly is done by using FASTX file and we can get the plot.
LIBRARY FORMATION IN SOLID:
(might download in the zipped format)